Journal: European Journal of Dentistry

Article Title: Interaction between TCF7L2 rs7903146 Genotype, HbA1c Levels, and the Periodontal Status of Dental Patients

doi: 10.1055/s-0041-1725578

Figure Lengend Snippet: The 2% agarose gel electrophoresis of RsaI digested PCR products. Line 1: 100 bp DNA ladder (Nippon Genetics Europe GmbH, Düren, Germany). Line 2: uncleaved PCR product. Line 3: C/C genotype. Line 4: C/T genotype. Line 5: T/T genotype. Line 6: negative control. PCR, polymerase chain reaction.

Article Snippet: Genotyping of TCF7L2 polymorphism rs7930146 was carried out by digesting the 188 bp PCR products in a reaction mixture with 10U of the restriction enzyme RsaI (Minotech Biotechnology, Heraklion, Crete, Greece), 1X RsaI Buffer (Minotech Biotechnology), and sterile ddH20 up to a final volume of 20 μL.

Techniques: Agarose Gel Electrophoresis, Negative Control, Polymerase Chain Reaction

Journal: Molecular Plant Pathology

Article Title: DCL‐suppressed Nicotiana benthamiana plants: valuable tools in research and biotechnology

doi: 10.1111/mpp.12761

Figure Lengend Snippet: Next‐generation sequencing (NGS) in DCLi and DCLi crossed plants. (A–C) Northern blot analysis of DCLi and DCLi crossed lines agroinfiltrated with green fluorescent protein (GFP) and GFPhp constructs; 21‐, 22‐ and 24‐nucleotide (nt) small RNAs were monitored. U6 was used as internal control. (D, E) Distribution of 20–24‐nt and 26–34‐nt small RNAs in wild‐type (WT), DCL1.13i, DCL2.11i, DCL3.10i, DCL4.9i, DCL1.13(x)2.11i, DCL2/4.5i and DCL3.10(x)2/4.5i. (F, H, I) 21–24‐nt micro‐RNA (miRNA) and trans‐acting small interfering RNA (tasiRNA) levels in the sequenced plant lines. (G) Validation of the bioinformatics analysis with quantitative polymerase chain reaction (qPCR) of miR159, miR166, miR168, miR396 and miR397. [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: For miRNA, 500 ng of DNAseI‐treated RNA (Roche Diagnostics) from four independent plants was reverse transcribed with RT polymerase (Minotech Biotechnology, Heraklion, Crete, Greece) using deoxyribonucleotide triphosphate(s) (dNTPs) (Invitrogen/Thermo Fisher Scientific, Waltman, Massachusetts, USA) and 0.5 μ m of the adequate reverse RT primer designed according to Chen et al. ( ) and Varkonyi‐Gasic et al. ( ) (Table , see Supporting Information).

Techniques: Next-Generation Sequencing, Northern Blot, Construct, Control, Small Interfering RNA, Biomarker Discovery, Real-time Polymerase Chain Reaction

Journal: Molecular Plant Pathology

Article Title: DCL‐suppressed Nicotiana benthamiana plants: valuable tools in research and biotechnology

doi: 10.1111/mpp.12761

Figure Lengend Snippet: Turnip mosaic virus (TuMV)‐infected DCL2/4i plants. Graph representing measurements with Quantity One 4.4.1 (Biorad) from three independent experiments of semi‐quantitative polymerase chain reaction (PCR) in DCL2/4i plants infected with TuMV for 1 or 2 weeks post‐infection (wpi). ‘ n ’ shows the number of individual tested plants. Student’s t ‐test was performed with * P < 0.05.

Article Snippet: For miRNA, 500 ng of DNAseI‐treated RNA (Roche Diagnostics) from four independent plants was reverse transcribed with RT polymerase (Minotech Biotechnology, Heraklion, Crete, Greece) using deoxyribonucleotide triphosphate(s) (dNTPs) (Invitrogen/Thermo Fisher Scientific, Waltman, Massachusetts, USA) and 0.5 μ m of the adequate reverse RT primer designed according to Chen et al. ( ) and Varkonyi‐Gasic et al. ( ) (Table , see Supporting Information).

Techniques: Virus, Infection, Real-time Polymerase Chain Reaction

Journal: Molecular Plant Pathology

Article Title: DCL‐suppressed Nicotiana benthamiana plants: valuable tools in research and biotechnology

doi: 10.1111/mpp.12761

Figure Lengend Snippet: DCL2 and DCL4 levels in Cucumber mosaic virus (CMV)‐, Tobacco rattle virus (TRV)‐ and Turnip mosaic virus (TuMV)‐infected DCL2/4i plants. Quantitative polymerase chain reaction (qPCR) experiments for the detection of endogenous DCL2 and DCL4 transcripts in CMV‐, TRV‐ or TuMV‐infected DCL2/4.5i and DCL2/4.16i plant lines at 2 weeks post‐infection (wpi).

Article Snippet: For miRNA, 500 ng of DNAseI‐treated RNA (Roche Diagnostics) from four independent plants was reverse transcribed with RT polymerase (Minotech Biotechnology, Heraklion, Crete, Greece) using deoxyribonucleotide triphosphate(s) (dNTPs) (Invitrogen/Thermo Fisher Scientific, Waltman, Massachusetts, USA) and 0.5 μ m of the adequate reverse RT primer designed according to Chen et al. ( ) and Varkonyi‐Gasic et al. ( ) (Table , see Supporting Information).

Techniques: Virus, Infection, Real-time Polymerase Chain Reaction